TABLE 1.

Stereological parameters used for estimating neuronal numbers in the white matter of the tree pangolin analyzed. CR, calretinin‐immunopositive neurons; NeuN, neuronal nuclear marker‐immunopositive neurons

Stain	Sectioning Plane	Counting frame size (µm)	Sampling grid size (µm)	Disector height (µm)	Section cut thickness (µm)	Measured mounted thickness (µm)	Upper and lower guard zones (µm)	Section interval	Number of sections	Number of sampling sites	Number of objects counted
NeuN	Coronal	150 × 150	600 × 600	20	50	26.4	2	80	11	486	957
CR	Coronal	150 × 150	600 × 600	18	50	23.2	2	40	11	383	247