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. 2022 Aug 3;258(2):179–188. doi: 10.1002/path.5988

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© 2022 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Feasibility of WGS in routine pathology practice. WGS was successfully completed in 854/1,216 (70%) samples containing tumor cells. The main reason for ineligibility for WGS was a low percentage of tumor cells in 24% of samples (9% insufficient pTCP and 15% insufficient mTCP) (A). Due to continuous optimizations during the course of the study, feasibility of WGS improved as samples with no tumor cells or insufficient TCP declined (B). WGS could be performed in a clinically acceptable time frame of a median of 12 working days. During the course of the study, the TAT decreased from 16 workings days in the first 3 months to 11 workings days in the last 3 months (C). Success rates of WGS procedures were high when using biopsy or resection specimens (70% and 77%, respectively), and could be performed both freshly obtained and fresh‐frozen archival tissue. Cytology specimens were less suitable for WGS, with a success rate of 21% (D). mTCP, molecular tumor cell percentage; pTCP, tumor cell percentage assessed by a pathologist.