Table 1.
Summary of published denture microbiome studies and their key characteristics
| Study | Location | Design | Controls (no. patient’s) | Cases (no. patient’s) | Extraction method | Storage | Sequencer | Region | Accession | Key findings |
|---|---|---|---|---|---|---|---|---|---|---|
| Campos et al. (2008) | São Paulo, Brazil | Cross‐Sectional | Healthy (10) | Denture stomatitis(10) | QuickExtract | NA | ABI PRISM 3100 Genetic Analyzer | 16S | AY672070‐76 | Distinct differences in microbiome between health and disease |
| O'Donnell et al. (2015) and Delaney et al. (2019)* | Glasgow, UK | Cross‐Sectional | Mucosa/plaque | Denture (130) | AGOWA mag Mini DNA Isolation Kit | −80°C | MiSeq | 16S V4, ITS1 | PRJNA324548 | Distinct associations between Candida load, oral hygiene and microbiome |
| Shi et al. (2016) | Los Angeles, USA | Cross‐Sectional | No denture stomatitis (10) | Denture stomatitis (10) | DNeasy Blood and Tissue kit | NA | 454 | 16S V1‐V3 | PRJNA292354 | Denture microbiome is reflective of that of teeth but identifiably distinct when comparing health and disease |
| Asakawa et al. (2018) | Fukuoka, Japan | Cross‐Sectional | Tongue (506) | Denture (137) | IsoQuick | −80°C | Ion PGM | 16S | DRA006979 | Diminished oral health and hygiene is reflected in the microbiota of the tongue |
| Yitzhaki et al. (2018) | Ramat‐Aviv, Israel | Cross‐Sectional | NA | Denture (26) | Genomic DNA Mini Kit | −20°C | MiSeq | 16S V3‐V4 | On request | Microbiome analysis suggests population distinction between olfactorily distinct samples |
| Nedumgottil (2018) | Puducherry, India | Cross‐Sectional | NA | Denture (88) | NucleoSpin Microbial DNA mini kit | NA | NA | 16S | On request | Denture wearers linked to co‐occurrence of Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens |
| Morse et al. (2019) | Cardiff, UK | Cross‐Sectional | No denture stomatitis (11) | Denture stomatitis (8) | Gentra PureGene Bact/Yeast DNA extraction kit | −20°C | MiSeq | 16S V1‐V3 | On request | Reduced bacterial diversity may lead to dysbiosis in DS |
| Mukai et al. (2020) | Yokohama, Japan | Cross‐Sectional | Saliva (8) | Denture (8) | ISOSPINE Fecal DNA Kit | −80°C | MiSeq | 16S V3‐V4 | PRJNA592277 | Microbial diversity diminished on denture surface compared to saliva |
| Murugesan et al. (2020) | Doha, Qatar | Cross‐sectional | Healthy (861) | Denture (136) | QIAsymphony | −80°C | MiSeq | 16S V1‐V3 | On request | Streptococcus and Neisseria found to be more abundant in denture wearers |
| Fujinami et al. (2021) | Nagoya, Japan | Cross‐Sectional | Plaque (16) | Denture (30) | MasterPure DNA Purification Kit | −20°C | MiSeq | 16S V3‐V4 | DRA011478 | Pathogens associated with aspiration pneumonia were more commonly isolated from dentures |
| Grischke et al. (2021) | Hannover, Germany | Cross‐Sectional | Healthy (372) | Peri‐implantitis/denture (725) | QIAshredder Mini Spin | −80°C | HiSeq | ND | PRJEB43417 | Removable dentures are identifiable as a risk‐factor for peri‐implantitis |
*
Two studies use same dataset for different analysis question.