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. 2022 Sep 26;119(40):e2212134119. doi: 10.1073/pnas.2212134119

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Copyright © 2022 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 6. — RPD3 deletion suppresses DBF4 deficiency. (A) Diploid strain YHy38 (FKH1/fkh1Δ DBF4/dbf4ΔC) was induced into meiosis and sporulation. Tetrads were dissected onto rich medium and incubated at 23 °C and imaged after 3 days and genotyped. Tetrads are arranged in columns; determined haploid genotypes are indicated on the panel to the right as F/f for FKH1/fkh1Δ and D/d for DBF4/dbf4ΔC; FKH1 and DBF4 are unlinked. Genotypes in red for inviable spores were inferred based on assumption of 2:2 segregation of alleles. The “fd” hypotheticals are circled. (B) Diploid strain OAy1188 (RPD3/rpd3Δ DBF4/dbf4ΔC) was induced into meiosis and sporulation. Tetrads were dissected onto rich medium and incubated at 30 °C and imaged and genotyped after 3 days. Tetrads are arranged in columns; determined haploid genotypes are indicated on the panel to the right as R/r for RPD3/rpd3Δ and D/d for DBF4/dbf4ΔC; RPD3 and DBF4 are unlinked. Genotypes in red for inviable spores were inferred based on assumption of 2:2 segregation of alleles. The “rd” isolates and hypotheticals are circled; the “Rd” hypotheticals are boxed.