Table 1.
Sequences of DNA oligonucleotides used in this study
| Oligonucleotides | Sequence |
|---|---|
| SIR2-deletion-F | ACATCTAGCACTCCTTCCAAC |
| SIR2-deletion-R | ACCTGCCCTTCTTACATTAAGC |
| RPD3-deletion-F | TCGCGGGCTGAACTGAATC |
| RPD3-deletion-R | GCTTTATCAACAGCGGTGGG |
| sir2-del-up | TTACTTGTAGCCTGCAACTCC |
| rpd3-del-up | TCAGCATAACGAATTGACGG |
| sir2-del-down | ATTCGACTTCTTTCCTTCGTTGT |
| rpd3-del-down | TGCAATTAGAAGAGAGTGAATC |
| int-sir2-down | ATCACAGGGTTCAATGTCGG |
| int-rpd3-down | TAGTGTTCAGTTGAATCACAC |
| SIR2-del-TRP-F | ATTCAAACCATTTTTCCCTCATCGGCACATTAAAGCTGGGACAAGTAACTGCAGGAATT |
| SIR2-del-TRP-R | ATATTAATTTGGCACTTTTAAATTATTAAATTGCCTTCTACACTATAGGGCGAATTGGGT |
| HIS-F | AGAATACCCTCCTTGACAGTC |
| HIS-R | TAGTATCGAATCGACAGCAG |
| HML-alpha-del-F | TATAGGGCAGTGTGTGACTTATGAATTGTTGTAGAAGGACgacatggaggcccagaatac |
| HML-alpha-del-R | ATGGCACAAGGAACACGCATTTTCCCAAGGCTTAGTATACcggcgttagtatcgaatcga |
| extend-HML-F | TTTTGGGACGATATTGTCATTATAGGGCAGTGTGTGACTT |
| extend-HML-R | TTTTATGAAGTAGCTTTCGGATGGCACAAGGAACACGCAT |
| probe-F | CTCACACTTGTACTCCATGA |
| probe-R | TGATGTGGAGAATAAGGTGC |
| 2xL-3xFLAG-FKH1-5′ | TTTCTACTACGACATCCATGGACGTAACAACAAACGCAAACGTGAACAATTCCTCTCTGAGTAGGGAACAAAAGCTGGAG |
| 2xL-3xFLAG-FKH1-3′ | TTCTTAACGGGTCTTTGTTCTTTATTGTTTAATAATACATATGGGTTCGACGACGCTGAATTCTATAGGGCGAATTGGGT |
| pAG61-Fkh1Δ5′ | AATAATAGTGTGTAAATTGTGCGTTCAATTAGCAAAGAAAgacatggaggcccagaatac |
| pAG61-Fkh1Δ3′ | TATTGTTTAATAATACATATGGGTTCGACGACGCTGAATTcggcgttagtatcgaatcga |
| Fkh2-up400bp | CATTACCGAAAATCTTCGATTTCGC |
| Fkh2-down375bp | CCGAAGCGTTGAGAAACAGC |
| Dbf4ΔC-F1 | AGCACAGACAGCACAGCCGGTGAAGAAAGAAACGGTAtgacggatccccgggttaattaa |
| Dbf4ΔC-R1 | GATTTTATCACTAAAAGCTACTGCACTTTACGTCGTGTCCcggcgttagtatcgaatcga |
| 3HA-MCM4-F | CGAGGGTGTAAGGAGATCAGTTCGCCTGAATAACCGTGTCCGGATCCCCGGGttaattaac |
| 3HA-MCM4-R | GATTTTATCACTAAAAGCTACTGCACTTTACGTCGTGTCCcggcgttagtatcgaatcga |
Oligonucleotides were obtained from International DNA Technologies.