Table 2.
Genotypes of S. cerevisiae strains used in this study
| Name | Genotype | Source |
|---|---|---|
| SSy161 | MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 bar1Δ::hisG | Viggiani et al., 2006 |
| CVy43 | ura3::BrdU-inc(URA3) | “ |
| CVy61 | trp1::BrdU-inc(TRP1) | “ |
| CVy68 | MATα leu2::BrdU-inc(LEU2) | “ |
| CVy44 | rpd3Δ::KanMX ura3::BrdU-inc(URA3) | Knott et al., 2009 |
| JHy3 | his3-1 leu2-0 ura3-0 met15-0 sir2Δ::KanMX | Mark Rose |
| JHy4 | his3-1 leu2-0 ura3-0 met15-0 rpd3Δ::KanMX | “ |
| PP1758 | fob1Δ::URA3 ura3::7xTK (URA3) rDNA20 (∼20copies) | Philippe Pasero |
| JPy115 | rpd3Δ::KanMX fob1Δ::URA3 ura3::7xTK (URA3) rDNA20 (∼20copies) | This study |
| MPy55 | FKH1-3xFLAG(TRP1) ADE2::FLOPv2x2 | “ |
| MPy102 | MCM4-3HA(KanMX) TRP1::HOPv1 | “ |
| MPy188 | fkh1Δ::URA3 | “ |
| OAy1096 | fkh1Δ::KanMX fkh2Δ::HIS3MX ars305Δ::BrdU-inc(URA3) | “ |
| OAy1100 | FKH1-3xFLAG(TRP1) | “ |
| OAy1102 | FKH1-3xFLAG(TRP1) ADE2::FLOPv1 | “ |
| OAy1107 | MATα fkh2Δ::URA3MX leu2::BrdU-inc(LEU2) | “ |
| OAy1109 | MATα fkh2-dsm leu2::BrdU-inc(LEU2) | “ |
| OAy1114 | fkh1Δ::KanMX fkh2-dsm ars305Δ::BrdU-inc(URA3) | “ |
| OAy1186 | MATα rpd3ΔKanMX leu2::BrdU-inc(LEU2) | “ |
| OAy1188 | OAy1186 x YHy19 diploid | “ |
| OAy1189 | dbf4ΔC::HIS3MX leu2::BrdU-inc(LEU2) | “ |
| YHy3 | sir2Δ::KanMX hmlɑΔ::URA3MX trp1::BrdU-inc(TRP1) | “ |
| YHy6 | sir2Δ::KanMX rpd3Δ::HIS3MX hmlɑΔ::URA3MX trp1::BrdU-inc(TRP1) | “ |
| YHy13 | fkh1Δ::KanMX fkh2-dsm rpd3Δ::HIS3MX ars305Δ::BrdU-inc(URA3) | “ |
| YHy17 | rpd3Δ::HIS3MX FKH1-3xFLAG(TRP1) ADE2::FLOPv1 | “ |
| YHy19 | dbf4ΔC::HIS3MX ura3::BrdU-inc(URA3) | “ |
| YHy27 | rpd3Δ::HIS3MX FKH1-3xFLAG(TRP1) ADE2::FLOPv2x2 | “ |
| YHy33 | sir2Δ::KanMX trp1::BrdU-inc(TRP1) | “ |
| YHy36 | his3-1 leu2-0 ura3-0 met15-0 rpd3Δ::HIS3MX | “ |
| YHy37 | rpd3Δ::HIS3MX MCM4-3HA(KanMX) TRP1::HOPv1 | “ |
| YHy38 | OAy1189 x MPy188 diploid | “ |
All strains are in the W303 (RAD5) background; the parental genotype of SSy161 is shown, whereas for other strains, only differences from this genotype are given, with the exceptions of strains JHy3, JHy4, and YHy36, which are in the BY4741 background and only used as source DNA for strain constructions.