Fig. 3.

Satb1-deficient CD4⁺ Tconvs and TCR75 cells from tolerant mice exhibit enhanced susceptibility to Treg suppression in vivo. (A) Experimental strategy. (B and C) Survival of B/c skin allografts transplanted into P14Rag^−/− recipients that received 4.5 × 10⁵ naive control or Satb1^−/− CD4⁺ Tconvs alone (in B) or without/with 2.25 × 10⁵ Foxp3^GFP+ Tregs ^+/− IL-2 (in C). n = 5 to 9/group; *P < 0.05, **P < 0.005, ***P ≤ 0.0005 (log-rank test). Curves from B are reproduced in C for comparison. (D and E) Experimental setting as in Fig. 1A. At day ≥ 35 postheart transplantation, TCR75 T cells isolated from spleen and lymph nodes of tolerant or rejector mice were CFSE-labeled and stimulated in vitro with soluble anti-CD3+B6 Rag^−/− splenocytes ^+/− Tregs for 3 d. CFSE dilution of TCR75 T cells was determined using flow cytometry. (D) Intrinsic proliferative capacity of TCR75 T cells in the absence of Tregs. (E) Percent suppression of TCR75 T cells in the presence of Tregs. n = 3 for memory and 4 for tolerant; *P < 0.05 (unpaired Student's t test).