Figure 3.

Autophagy markers determined via immunoblotting. (a) Expression of two autophagy markers, namely, p62 and LC3, in THP-1 cells after their engulfment of RBCs is determined with immunoblotting. The rapamycin group is the autophagy positive control. BafA1 is used as the autophagy inhibitor. (b)–(d) Histograms illustrating the relative amounts of p62 and LC3 II to GAPDH and those between LC3 II and LC3 I. (e)–(m) Relative amounts of p62 and LC3 II of THP-1 cells coincubated with three different RBCs with or without bafromycin. ^∗p < 0.05, ^∗∗p < 0.01, and ns: not significant.