Figure 6.

NLRP3 and GSDMD determined via immunoblotting. (a) The expressions of NLRP3 and GSDMD determined via immunoblot. (b) Histograms of the NLRP3 activation state of three different THP-1 cells after receiving the same stimulation in nine groups. (c) Relative quantity histograms of GSDMD of three different THP-1 cells after receiving the same stimulation in nine groups. (d)–(f) Histograms of the NLRP3 relative amounts of wild-type, shNC, and ID3 THP-1 cells after phagocytosis of erythrocytes. (g)–(i) Regulatory effect of BafA1 on NLRP3 expression. (j)–(l) Histograms of the GSDMD relative amounts of wild-type, shNC, and ID3 THP-1 cells after phagocytosis of RBCs. (m)–(o) Regulatory effect of BafA1 on GSDMD expression. ∗p < 0.05, ∗∗p < 0.01, and ns: not significant.