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. 2022 Sep 30;2022:3627026. doi: 10.1155/2022/3627026

Figure 2.

Figure 2

Hypoxic exposure reduces the amount of CIRBP. (a) Western blot results indicated CIRBP expression levels in C17.2-NSCs at 24, 36, and 48 hours following hypoxic exposure. (b) Quantification of CIRBP expression levels in C17.2-NSCs showed insignificant change following 24 hours of hypoxic exposure; the CIRBP level was gradually reduced after 36 h and 48 h of hypoxic exposure, n = 3. (c) Representative fluorescence photographs suggested the expression level of CIRBP (green) and DAPI (blue) at 48 h hypoxic exposure in C17.2-NSCs. Bars = 50 μm. (d) Quantitative analysis of the percentage of CIRBP+ C17.2-NSCs in each sight. The percentage of CIRBP+ C17.2-NSCs was significantly reduced 48 h after hypoxic treatment, n = 6. Differences between hypoxia and normoxia (21% O2) were indicated with asterisks.