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. 2022 Sep 23;13:893914. doi: 10.3389/fimmu.2022.893914

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Copyright © 2022 Ye, Zhang, Zhang, Luan, Xu, Wu, Ju and Hu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A), Immunoblot analysis of NLRP3, GSDMD and GSDMD-NT protein expression in adult primary cardiomyocytes subjected to hypoxia for 6 hours and reoxygenation for 12 hours. (B), Representative images of Ana-1 cells and J774a.1 cells after different treatments were acquired by microscopy. (C), Representative Western blots showing NLRP3, GSDMD, GSDMD-NT, mature IL-1β and cleaved caspase-1 in Ana-1 cells treated with hypoxia for 6 hours and reoxygenation for 1 hour. (D), Representative Western blots showing GSDMD, GSDMD-NT in J774a.1 cells treated with hypoxia and reoxygenation. (E), The level of LDH released in the supernatant from Ana-1 cells after H/R. (* indicated p<0.05).