Fig. 2.
Assay optimization of the important parameters for SARS-CoV-2 DNA based detection including: (A) acpcPNA concentrations; (B) hybridization times; (C) detection potentials for measuring 50 nM SARS-CoV-2 DNA (N gene) via amperometric technique at 120 s sampling times; (D) the amperometric current response for 5 mM [Fe(CN)6]3-/4- in presence (S) and in absence (B) of 50 nM SARS-CoV-2 target. The error bar of all experiment represented the standard deviation of three repetitive measurements using three independent devices (n = 3).