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. 2022 Oct 7;11(1):60. doi: 10.1038/s41389-022-00435-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A BioChIP-seq signals at the inhibitor of DNA binding 3 (ID3) locus in MDA-MB-231 cells expressing BirA alone or BirA and FB-ZNF148. B RT-qPCR analysis for ZNF148 (left panel, n = 6), ID3 (middle panel, n = 12) and ID1 (right panel, n = 6) mRNA transcripts, relative to GAPDH and shControl (shCtr), in shZNF148 MDA-MB-231 cells. C RT-qPCR analysis for ZNF148 (left panel, n = 6), ID3 (middle panel, n = 4) and ID1 (right panel, n = 9) mRNA transcripts, relative to GAPDH and empty vector (EV) control, in ZNF148 overexpressing MDA-MB-231 cells. D Scatter plots of ID3 vs. ZNF148 (Pearson r = −0.3776, p < 0.0001), and ID1 vs. ZNF148 (Pearson r = −0.1761, p < 0.0001) mRNA levels (log2) from 1904 breast cancer patient samples in METABRIC cohort [56, 78]. E Scatter plot of ID3 vs MYC and ID1 vs MYC as in “D”, with Pearson r = 0.3359 (p < 0.0001) and Pearson r = 0.1590 (p < 0.0001) respectively, indicating a direct correlation of ID3/1 and MYC mRNA levels. F Schematic diagram of ZNF148 actively repressing ID1/3 gene expression. G Schematic diagram of regulatory circuitry between MYC, ZNF148, and ID1/3 in MYC-driven stem cell-like cancer. Student’s t-test, ***P < 0.001. ****P < 0.0001. Error bars indicate ± SD.