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. 2022 Oct 7;12:16899. doi: 10.1038/s41598-022-21345-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MMP-1 was essential for CAMSAP2-induced migration and invasion in colorectal cancer cells. (A) Western blot analysis showed that MMP-1 was silenced by shRNA against MMP-1 in CAMSAP2-overexpessing SW-620 cells. (B) Wound healing scratch assay showed ectopic expression of MMP-1 attenuated CAMSAP2 shRNA-reduced migration in SW-620 and SW-480 cells. Left: representative images of wound scratch. Right: histograms represent the analysis of the wound healing rate. (C) Transwell migration assay showed overexpression of MMP-1 attenuated the inhibition of CAMSAP2 shRNA on colorectal cancer cell migration. (D) Enforced overexpression of MMP-1 restored the invasion-attenuated by CAMSAP2 shRNAs in colorectal cancer cells. (E) Western blot analysis of MMP-1 expression in CAMSAP2-silenced SW-620 and SW-480 cells transduced with MMP-1 plasmid (MMP-1) or empty vector (Vector). (F) Wound healing assay showed silencing MMP-1 attenuated CAMSAP2-induced migration in colorectal cancer cells. Left: representative images of wound scratch. Right: histograms represent the analysis of the wound healing rate. (G) Transwell migration assay showed knockdown of MMP-1 attenuated the CAMSAP2-induced migration in SW-620 cells. (H) Knockdown of MMP-1 inhibited the colorectal cancer cell invasion induced by CAMSAP2 overexpression. *P < 0.05; **P < 0.01; ***P < 0.001.