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. 2022 Oct 7;12:16899. doi: 10.1038/s41598-022-21345-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CAMSAP2 enhanced the expression of MMP-1 through activation of JNK/c-Jun signaling pathway. (A) Western blot analysis of p-JNK (T183/Y185), JNK, p–c-Jun (Ser73) and c-Jun in CAMSAP2 expressing or depleted colorectal cancer cells. (B) Targeting JNK by SP600125 reduced the expression of MMP-1 induced by CAMSAP2 in colorectal cancer cells. (C) Representative luciferase reporter assay from colorectal cancer cells transfected with an MMP-1 luciferase reporter, in the presence or absence of SP600125 (10 μM). Promoter activity was measured using a dual-luciferase system. Data are presented as relative to the DMSO control. (D) ChIP analysis of c-Jun occupancy on the promoter of MMP-1 in SW-620 cells. Fold enrichments normalized to IgG are presented. The values are means and SDs of three independent experiments. (E,F) Transwell migration (E) and invasion (F) assay revealed that SP600125 attenuated the migration and invasion induced by CAMSAP2 overexpression in colorectal cancer cells. (G) Western blot analysis of c-Jun and MMP-1 in CAMSAP2-depleted SW-620 cells transduced with c-Jun plasmid or empty vector. (H) Luciferase reporter assay revealed that ectopic expression of c-Jun restored the transcription activity of MMP-1-reduced by CAMSAP2 shRNAs in SW-620 cells. Promoter activity was measured using a dual-luciferase system. Data are presented as relative to the control group (shNC + Vector). (I) ChIP experiment showed that knockdown of CAMSAP2 led to loss of recruitment of c-Jun on the MMP-1 gene promoter of in SW-620 cells. (J,K) Transwell migration (J) and invasion (K) assay showed that enforced expression of c-Jun rescued the migration and invasion attenuated by CAMSAP2 shRNAs in SW-620 cells. *P < 0.05; **P < 0.01; ***P < 0.001.