Fig. 1. Overview of experimental approach to assess the impact of microenvironmental factors.

a Map of LINCS data generation and analysis centers. b Schematic illustrating the experimental and analytical approaches to link molecular and cellular phenotypes. c Schematic of the experimental design, cell culture protocol, and sample harvest time points. d The experimental treatments, dosages, and assays deployed to generate the LINCS ME perturbation datasets. e Summary of the assays, time points, and features for the three experimental collections.