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. 2022 Oct 7;5:1066. doi: 10.1038/s42003-022-03975-9

Fig. 2. Ligand treatments induce diverse phenotypic responses.

Fig. 2

a Representative immunofluorescent images of ligand-induced cellular phenotypes at 48H. MCF10A cells were stained with Cell Mask to visualize cytoplasm. b Cartoon showing the image-based cellular phenotypes assessed from the immunofluorescence and live cell imaging assays. c–g Boxplots summarizing cellular phenotypes at time 0H (CTRL) and 48H after ligand addition from 8 biological replicates. Individual datapoints represent well-level means normalized to 0H. Circles are from collection 1 and triangles are from collection 2. The interquartile range is indicated by the box, with whiskers extending to no further than 1.5 times the interquartile range. Note that EdU positive proportion was not measured at 0H. Data in Supplementary Data 1. h Accumulated cell migration (colored lines) from 0-48H for 25 cell lineages (individual cells and one of their progeny if they divided). Circles indicate mitotic events. The solid black lines indicate the population average; the dotted gray line shows the average TGFB + EGF induced migration at 48H, which was the treatment that induced the greatest increase in cell migration. Data in Supplementary Data 2, 3.