TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Bartonellae | ||
| JK9R | B. henselae; human isolate from cutaneous bacillary angiomatosis lesion | J. E. Koehler |
| JK31 | B. quintana; human isolate from cutaneous bacillary angiomatosis lesion | J. E. Koehler |
| BBL | B. bacilliformis; human isolate from blood | Generous gift of Robert Gilman, Lima, Peru |
| V. cholerae | ||
| MBG40 | O395 irgA::TnphoA Smr Kmr | 20 |
| CML13 | O395 irgA::TnphoA fur::pCML13 Smr Kmr Apr | 20 |
| Plasmids | ||
| pANT3 | oriRSF1010 mob+ Kmr Smr; promoterless gfpmut3 | 18 |
| pSYP1 | pANT3 backbone; putative Bartonella fur promoter upstream from gfpmut3 | This study |
| pSYP2 | pANT3 backbone; inverted putative Bartonella fur promoter upstream from gfpmut3 | This study |
| pANT4 | oriRSF1010 bla+mob+laclq::Kmr; ptac promoter upstream from gfpmut3 | 18 |
| pSYP3 | pANT4 backbone; ptac promoter upstream from B. henselae fur | This study |
| pACYC184 | orip15A Cmr Tcr | New England BioLabs, Inc., Beverly, Mass. |
| pSYP4 | pACYC184 backbone; ptac promoter upstream from B. henselae fur cloned in place of tet gene | This study |