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. 2022 Oct 7;12:16885. doi: 10.1038/s41598-022-21506-8

Figure 1.

Figure 1

Characterization of vaccinia VG9/(SST-14)2-HSA. (A) Schematic illustration of VG9/(SST-14)2-HSA construction. Somatostatin fusion protein (SST-14)2-HSA expression cassette was inserted into TK locus of VG9 strain via homologous recombination. In the expression cassette, gpt gene is driven by the promoter P-7.5 K in cis direction while SST14-HSA is driven by the reverse promoter P-se/I. (B) Western blot analysis of the immunogenicity of HSA and somatostatin in different vaccinia VG9/(SST-14)2-HSA recombinants. The blots were cut prior to hybridization with the antibody to control protein of β-actin during blotting.