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Collection and digestion of genetically modified organoids
(A) Representative scheme summarizing the multistep process of preparation and digestion of genetically modified organoids prior to transplantation.
(B) Representative microscopic images of ∼70% confluent APTKA organoids prior to collection and Accutase-dissociated organoids resuspended in 20% Matrigel/PBS. Scale bars represent 200 μm.
