APTKA organoids culture medium

Reagent	Final concentration	Amount
Ad-DF +++	N/A	38.532 mL
B-27 supplement (50×)	1×	800 μL
N-2 supplement (100×)	1×	400 μL
N-Acetyl-L-cysteine	2 mM	100 μL
Puromycin dihydrochloride	2 μg/mL	8 μL
Hygromycin	200 μg/mL	160 μL
Total	N/A	40 mL

Once prepared, stored at 4°C for 4 weeks.