Antibodies |
|
Rabbit polyclonal anti-Fibronectin |
Abcam |
Cat# ab2413; RRID:AB_2262874 |
Mouse monoclonal anti-Collagen I |
Abcam |
Cat# ab90395; RRID:AB_2049527 |
Rabbit polyclonal anti-Collagen IV |
Abcam |
Cat# ab6586; RRID:AB_305584 |
Rabbit polyclonal anti-Laminin |
Abcam |
Cat# ab11575; RRID:AB_298179 |
Rat polyclonal anti-Ki67 |
eBioscience |
Cat# 14-5698-82; RRID:AB_10854564 |
Goat anti-Rabbit IgG Alexa Fluor™ 594 |
ThermoFisher |
Cat# A-11037; RRID:AB_2534095 |
Goat anti-Mouse IgG Alexa Fluor™ 594 |
ThermoFisher |
Cat# A-11032; RRID:AB_2534091 |
Goat anti-Rat IgG Alexa Fluor™ 488 |
ThermoFisher |
Cat# A-11006; RRID:AB_141373 |
Normal Goat Serum |
ThermoFisher |
PCN5000 |
|
Chemicals, peptides, and recombinant proteins |
|
Sodium deoxycholate |
Sigma Aldrich |
30970 |
DNase I, Bovine Pancreas |
Millipore |
260913 |
Paraformaldehyde |
Sigma Aldrich |
158127 |
Tissue-Tek® O.C.T. Compound |
Sakura Finetek |
4583 |
Triton™ X-100 |
Sigma Aldrich |
X100 |
Bovine Serum Albumin |
Sigma Aldrich |
A7030 |
TRIS buffer saline |
Sigma Aldrich |
T5912 |
OmniPur® Methylene Blue, Trihydrate |
Sigma Aldrich |
6210-OP |
|
Critical commercial assays |
|
SPECTRA H&E Stains |
Leica |
3801654 |
DNeasy Blood & Tissue Kit |
Qiagen |
69504 |
QuickZyme Total Collagen Assay kit |
QuickZyme Biosciences |
QZBtotcol |
Glycosaminoglycan Assay Blyscan™ |
Biocolor |
B1000 |
|
Experimental models: Organisms/strains |
|
Male and female pigs Sus scrofa domesticus
|
JSR Genetics Ltd |
N/A |
Male New Zealand white rabbits Oryctolagus cuniculus
|
Charles River Laboratories |
N/A |
|
Software and algorithms |
|
GraphPad Prism version 6.05 |
GraphPad Software Inc. |
https://www.graphpad.com/ |
BioRender |
BioRender |
https://biorender.com/ |