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The negative effect of exogenous K+ addition on KdpB levels in Anabaena. Cultures were grown in BG-11/K5 (lane 1) or BG-11/K0 (lane 2) medium for 2 days. On day 2, 5 mM KCl was added to part of the BG-11/K0 culture at time zero. Samples were removed 0.5 (lane 3), 2 (lane 4), 4 (lane 5), or 8 (lane 6) h after K+ addition, electrophoretically resolved for a longer duration, and processed for KdpB immunodetection as for Fig. 2B.
