Table 3.
Immunohistochemistry staining protocols
| Target marker | Antibody source | Antibody dilution |
|---|---|---|
| WNT5A | Rabbit polyclonal IgG antibody (ab229220; Abcam, Cambridge, UK) | 1:100 |
| N-Cadherin | Rabbit monoclonal IgG antibody (#13116S; Cell Signaling Technology, Danvers, MA, USA) | 1:125 |
| Vimentin | Mouse monoclonal IgG antibody (ab8978; Abcam) | 1:100 |
| α-SMA | Mouse monoclonal IgG antibody (ab7817l; Abcam) | 1:100 |
| E-Cadherin | Mouse monoclonal IgG antibody (#14472S; Cell Signaling Technology) | 1:100 |
WNT5A Wnt family member 5A, α-SMA smooth muscle actin