FIG. 2.

PCR amplification of IVSs and rRNA fragmentation in strains of enteric bacteria. Ec, E. coli K-12; Stm, S. enterica serovar Typhimurium LT2; Cam, C. amalonaticus SA5617 (lane 3) and SA5664 (lane 4); Kox, K. oxytoca SA5365; Pst, P. stuartii ATCC 33672. (A) Agarose gel containing PCR products from amplification of regions of rrl genes containing IVSs in helix 25 or helix 45; after PCR they were separated by electrophoresis in a gel containing ethidium bromide and visualized under UV light. (B) rRNA was blotted on a Hybond-N+ membrane and stained with methylene blue.