Table 1.
Characteristics and screening conditions for the different viruses tested against the PCL compounds. The viruses differ in their genome composition, size, envelopment, tropism, and plaque shape. The screening conditions were optimized for each virus individually. FFU = focus forming unit, GFP = green fluorescent protein, NP = nucleoprotein, BafA1 = Bafilomycin A1, GCV = Ganciclovir.
| RV-A16 | IAV-H1N1 (WSN) | HSV-1 | ||
|---|---|---|---|---|
| Characteristics | Genome | Linear (+)ssRNA | Segmented (−)ssRNA | Linear dsDNA |
| Genome/Virion | 7.2 kb/30 nm | 13.5 kb/100 nm | 152 kbp/130 nm | |
| Envelope | no | yes | yes | |
| Tropism | Upper respiratory tract | Respiratory tract | Epithelial mucosa, neuronal cells | |
| Screening conditions | Read-out | GFP | α-NP antibody staining | GFP |
| Plaque shape | Round, comet | Comet | Round, comet | |
| Positive control | 0.6 nM BafA1 | 1.5 nM BafA1 | 25 µM Ganciclovir | |
| Negative control | 0.0125% DMSO | 0.0125% DMSO | 0.025% DMSO | |
| Number of cells per well seeded | 6,000 HOG | 10,000 A549 | 8,000 A549 | |
| Inoculation FFU per well | 30 | 25 | 50 | |
| Infection time | 48 h | 24 h | 26 h | |
| Infection temperature | 33.5 °C | 37 °C | 37 °C |