. 2022 Oct 8;9:610. doi: 10.1038/s41597-022-01733-4

Table 2.

Representation of the Z’-factor plates. To assess the quality of the screening platform, Z’-factors were determined in dedicated plates before conducting the drug screens. For each virus, two plates with 192 technical replicates of each positive and negative control were infected, and Z’-factors determined for the five screen read-outs by independent imaging at UZH and EPFL (RV and HSV-1 only at UZH) using the formula given in Eq. (1) for 3σ and 2σ.

RV	Plate	UZH					UZH
Barcode		3 sigma					2 sigma
Barcode		numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity
BSF019007	Za	−12.59	0.50	0.51	0.53	0.49	−8.06	0.67	0.67	0.68	0.66
Mean		−12.59	0.50	0.51	0.53	0.49	−8.06	0.67	0.67	0.68	0.66
IAV		UZH					UZH
Barcode	Plate	3 sigma					2 sigma
Barcode	Plate	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity
BSF019088	Za	−0.15	0.14	0.14	0.32	0.30	0.23	0.42	0.43	0.55	0.53
BSF019089	Zb	−0.22	0.18	0.13	0.42	0.38	0.18	0.46	0.42	0.61	0.58
Mean		−0.19	0.16	0.13	0.37	0.34	0.21	0.44	0.42	0.58	0.56
		EPFL					EPFL
Barcode	Plate	3 sigma					2 sigma
Barcode	Plate	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity
BSF019088	Za	−0.02	0.39	0.36	0.42	0.41	0.32	0.59	0.57	0.61	0.61
BSF019089	Zb	−0.08	0.36	0.30	0.50	0.50	0.28	0.58	0.53	0.67	0.66
Mean		−0.05	0.37	0.33	0.46	0.45	0.30	0.58	0.55	0.64	0.64
HSV		UZH					UZH
Barcode	Plate	3 sigma					2 sigma
Barcode	Plate	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity	numberOfNuclei	numberOfInfectedNuclei	infectionIndex	numberOfPlaques	totalVirusIntensity
BSF020768	Za	−8.32	0.04	0.20	0.37	−0.01	−5.21	0.36	0.47	0.58	0.32
BSF020769	Zb	−23.01	0.25	0.21	0.38	0.21	−15.01	0.50	0.47	0.59	0.47
Mean		−15.67	0.15	0.20	0.37	0.10	−10.11	0.43	0.47	0.58	0.40