Table 4.

Top hits with effects on RV, IAV, or HSV infection. The summary includes positive and negative controls. A compound was labelled toxic if scored as such by the PrestoBlue toxicity assay or by the Z-score for the parameter ‘nuclei’ in the screens. Mean read-outs were calculated by averaging four biological replicates (three for HSV) for PCL compounds, and 32 technical replicates over four biological replicates (three for HSV) for positive and negative controls. Normalized values are mean read-outs relative to the mean of the corresponding positive control over all replicates. Compounds that showed low toxicity and high inhibitory or enhancing effects on viral infection were selected as best hits depending on their clinical potential.

Virus	PCL_ID	compoundIdentifier	Compound	Group	Toxic		Mean read-outs					Normalized mean read-outs					Hit
						Analysis	Nuclei	Infected nuclei	Infection index	Plaques	Virus intensity [AU]	Nuclei	Infected nuclei	Infection index	Plaques	Virus intensity [AU]	Nuclei	Infected nuclei	Infection index	Plaques	Virus intensity [AU]	Effect
RV	DMSO	DMSO	DMSO	neg. ctr.	no	UZH	10,822	2,828	0.26	30.04	4.21E + 09	1.00	1.00	1.00	1.00	1.00	—	—	—	—	—	—
						EPFL	5,547	1,440	0.29	62.64	1.42E + 09	1.00	1.00	1.00	1.00	1.00
	BafA	BafA	BafA	pos. ctr.	no	UZH	9,096	69	0.01	0.50	3.04E + 09	0.84	0.02	0.03	0.02	0.72	—	—	—	—	—	Inhibitor
						EPFL	4,471	10	0.00	0.38	9.71E + 08	0.41	0.00	0.01	0.01	0.23
	Prestw-1717	452	Aminacrine	PCL	yes	UZH	8,145	415	0.05	0.25	3.11E + 09	0.75	0.15	0.19	0.01	0.74	no	yes	yes	yes	yes	Inhibitor
						EPFL	4,768	188	0.04	1.50	1.01E + 09	0.44	0.07	0.16	0.05	0.24	no	yes	yes	yes	yes
	Prestw-388	1157	5-Azacytidine	PCL	yes	UZH	9,195	672	0.07	6.00	3.28E + 09	0.85	0.24	0.27	0.20	0.78	no	yes	yes	yes	yes	Inhibitor
						EPFL	6,015	295	0.05	3.25	1.06E + 09	0.56	0.10	0.19	0.11	0.25	no	no	no	no	no
	Prestw-787	1078	Merbromin	PCL	no	UZH	12,267	7,148	0.58	47.25	5.81E + 09	1.13	2.53	2.22	1.57	1.38	no	yes	yes	yes	yes	Enhancer
						EPFL	6,035	1,511	0.29	64.75	1.45E + 09	0.56	0.53	1.09	2.16	0.34	no	no	no	no	no
IAV	DMSO	DMSO	DMSO	neg. ctr.	no	UZH	9,044	1,746	0.19	24.66	4.45E + 09	1.00	1.00	1.00	1.00	1.00	—	—	—	—	—	—
						EPFL	7,977	1,348	0.17	33.66	1.74E + 09	1.00	1.00	1.00	1.00	1.00
	BafA	BafA	BafA	pos. ctr.	no	UZH	7,782	17	0.00	0.34	2.95E + 09	0.86	0.01	0.01	0.01	0.66	—	—	—	—	—	Inhibitor
						EPFL	7,076	11	0.00	0.38	1.25E + 09	0.89	0.01	0.01	0.01	0.72
	Prestw-267	631	Clotrimazole	PCL	no	UZH	9,173	481	0.05	10.75	3.36E + 09	1.01	0.28	0.28	0.44	0.75	no	yes	yes	yes	yes	Inhibitor
						EPFL	7,432	410	0.06	12.25	1.39E + 09	0.93	0.30	0.33	0.36	0.80	no	no	no	no	no
	Prestw-491	850	Metixene hydrochloride	PCL	no	UZH	8,213	2,008	0.24	26.00	5.25E + 09	0.91	1.15	1.26	1.05	1.18	no	no	no	no	yes	Enhancer
						EPFL	7,838	1,571	0.20	38.25	1.80E + 09	0.98	1.17	1.17	1.14	1.03	no	no	no	no	no
HSV	DMSO	DMSO	DMSO	neg. ctr.	no	UZH	22,286	10,532	0.49	66.06	4.11E + 09	1.00	1.00	1.00	1.00	1.00	—	—	—	—	—	—
	GCV	GCV	GCV	pos. ctr.	no	UZH	20,270	475	0.02	0.77	1.50E + 09	0.91	0.05	0.05	0.01	0.37	—	—	—	—	—	Inhibitor
	Prestw-1717	452	Aminacrine	PCL	no	UZH	18,402	4,467	0.24	39.33	3.06E + 09	0.83	0.42	0.50	0.60	0.75	yes	yes	yes	yes	yes	Inhibitor
	Prestw-1793	525	Raltitrexed	PCL	no	UZH	16,108	3,562	0.22	35.33	2.67E + 09	0.72	0.34	0.45	0.53	0.65	yes	yes	yes	yes	yes	Inhibitor
	Prestw-655	963	Halcinonide	PCL	no	UZH	15,912	13,749	0.85	83.67	5.45E + 09	0.71	1.31	1.75	1.27	1.33	yes	no	yes	no	yes	Enhancer
	Prestw-781	1072	Clobetasol propionate	PCL	yes	UZH	16,267	14,587	0.89	85.67	5.31E + 09	0.73	1.39	1.82	1.30	1.29	yes	yes	yes	yes	yes	Enhancer