Table 2.

Immunohistochemical Quantitative Assay of P53

Experimental Groups N=8	Immunopositively Cells (Mean %)**	IHC Scoring Intensity (1–100%)	IHC Scaling Intensity (0–4)	IHC Grading	Final Grading System
(G1) CNG	20.68%A	1–25%	1	Weak	+
(G2) CPG	78.39%D	75–100%	4	Very Strong	++++
(G3) OXA+ ZIN 15 mg/kg	57.81%C	50–75%	3	Strong	+++
(G4) OXA+ MEL 10 mg/kg	44.27%B	25–50%	2	Moderate	++
(G5) OXA+ ZIN+ MEL (PG)	21.96%A	1–25%	1	Weak	+
(G6) OXA+ ZIN+ MEL (TG)	23.31%A	1–25%	1	Weak	+

Notes: **Each value represents the mean percentage of immunopositively cells from eight animals (n=8). Statistical comparison among groups: Mean values with different capital letters (A, B, C, and D) have significant differences at (P < 0.05).

Abbreviations: G1, negative control group (CNG); G2, positive control group (CPG); G3, positive control group with zinc (ZIN); G4, positive control group with melatonin (MEL); G5, positive control group with zinc and melatonin, preventive approach group (PG); G6, positive control group with zinc and melatonin, treatment approach group (TG).