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. 2022 Sep 7;144(5):1027–1048. doi: 10.1007/s00401-022-02489-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Mitochondrial localization of H3.3G34R. A Enrichment of subcellular localizations (Gene Ontology Cellular Compartments) among the proteins differentially bound by each oncohistone. B Relative enrichment [log2(H3/control)] of outer (TOMM) and inner (TIMM) mitochondrial transporter proteins associated with each histone. p: t test. C Confocal microscopy of NHA cells expressing H3.3G34R-FLAG/HA and stained with MitoTracker Red and HA. Box shows zoom area at right. Arrows mark co-localization of H3.3G34R with mitochondria. Scale bar: 3 μm. D Quantification of colocalization between MitoTracker Red and H3.3 in NHA cells expressing H3.3WT or H3.3G34R (antibody: HA tag) or 7316-158 cells (antibody: H3.3G34R). Bars show mean ± standard error of at least 7 fields of view. E Co-immunoprecipitation of H3-FLAG/HA and TFAM from NHA cells transduced with indicated constructs and subjected to HA IP. F Principal components analysis of normalized metabolite concentrations in NHAs expressing H3.3WT or H3.3G34R as determined by LC–MS (n = 4). G Enriched metabolic pathways in H3.3G34R vs H3.3WT NHAs. H Relative concentration of indicated TCA metabolites in H3-expressing NHA cell lines (n = 4). p: t test