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. 2022 Sep 19;144(5):881–910. doi: 10.1007/s00401-022-02491-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Reduced psyn129 in M83+/+ mice carrying STI1 hypomorphic alleles. a Immunoblot from RIPA extracted striatal lysates from M83+/+:ΔTPR1 mice. b Densitometric quantification of psyn129 (ab51253) antibody, relative to total human α-synuclein (genotype: F(2,11) = 2484, p < 0.0001, adjusted for multiple comparisons with Dunnett’s multiple comparisons: M83+/+:STI1WT vs. M83+/+:ΔHET p = 0.005, M83+/+:STI1WT vs. M83+/+:ΔTPR1 p < 0.0001) c Human α-synuclein relative to actin loading control (genotype: F(2,11) = 2.887, p = 0.098). d Immunoblot from RIPA cortical lysates and e. Densitometric quantifications of psyn129 (ab51253) relative to total human α-synuclein (genotype: F(2,11) = 20.57, p = 0.0002, adjusted for multiple comparisons with Dunnett’s multiple comparisons test: M83+/+:STI1WT vs. M83+/+:ΔHET p = 0.305, M83+/+:STI1WT vs. M83+/+:ΔTPR1 p = 0.0001) f Densitometric quantification of human α-synuclein relative to actin loading control (genotype: F(2,11) = 2.048, p = 0.175). g Representative immunoblot for STI1, psyn129, and human α-synuclein in M83+/+ :ΔTPR1 mice in SDS/Urea-soluble cortical fraction (RIPA-insoluble fraction). h Densitometric quantification of psyn129 (relative to human α-synuclein; genotype, F(2,11) = 106, p = 0.0009, adjusted for multiple comparisons with Dunnett’s multiple comparisons: M83+/+:STI1WT vs. M83+/+:ΔHET p = 0.619, M83+/+:STI1WT vs. M83+/+:ΔTPR1 p = 0.0008). i Densitometric quantification of human α-synuclein (relative to amido black) (genotype, F(2,11) = 19.49, p = 0.0002, Dunnett’s multiple comparisons: M83+/+:STI1WT vs. M83+/+:ΔHET p = 0.868, M83+/+:STI1WT vs. M83+/+:ΔTPR1 p = 0.0003). j Immunoblot for RIPA brainstem lysates and k Densitometric quantifications of psyn129 (ab51253) relative to total human α-synuclein (genotype F(2,10) = 14.93, p = 0.001, Dunnett’s multiple comparisons test: M83+/+:STI1WT vs. M83+/+:ΔHET p = 0.0024, M83+/+:STI1WT vs. M83+/+:ΔTPR1 p = 0.0013). l Densitometric quantifications of human α-synuclein relative to actin loading control (genotype F(2,10) = 25.60, p = 0.0001, Adjusted for multiple comparisons with Dunnett’s multiple comparisons test: M83+/+:STI1WT vs. M83+/+:ΔHET p = 0.0594, M83+/+:STI1WT vs. M83+/+:ΔTPR1 p = 0.0012). m Immunoblots from SDS/Urea-soluble brainstem tissue lysates. n densitometric quantification psyn129 (relative to human α-synuclein) (genotype, F(2,10) = 1.827, p = 0.217). o. Densitometric quantification of human α-synuclein (relative to amido black) (genotype (F(2,10) = 0.9151, p = 0.4316). N = 4–5 mice per genotype. Individual dark blue circles (M83+/+:STI1WT), half-filled pink triangles (M83+/+:ΔHET), or filled turquoise triangles (M83+/+:ΔTPR1) represent individual animals. White open circle represents M83 non-transgenic mice (WT mice). Immunoblotting data presented as mean ± SEM and analyzed using one-way ANOVA with appropriate post hoc comparisons, when required. *p < 0.05, **p < 0.01, ***p < 0.0001