Figure 6. ApoA-I inhibits thrombosis in mice and SR_BI is necessary for apoA-I inhibition.

A. Time (minutes) to vessel occlusion in a 6% ferric chloride-induced carotid artery injury model with vehicle control-treated WT mice (black bar, n=5) compared to those treated with apoA-I (gray bar). ApoA-I preincubation significantly prolonged the time to vessel occlusion versus NS-pretreated control mice (n=5; *=p<0.05). B. Survival time (minutes) following pulmonary embolism induced by intravenous injection of collagen and epinephrine in mice treated with vehicle control (black, n=5) or apoA-I (red, n=5). All the NS-treated controls died via a pulmonary embolus within 5 minutes following injury. ApoA-I pre-treated mice survived and did not develop pulmonary emboli during the observation period. Statistical significance determined by Mann-Whitney test; *=p<0.05. C. Maximal aggregation (%) was compared between experimental groups with no difference seen in SR-BI KO mice treated with apoA-I (n=3) or vehicle (normal saline, n=3), but significant decrease in aggregation seen in WT mice treated with apoA-I (n=4) compared to controls treated with vehicle only (n=7, *=p<0.05).