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Overview of the experimental design. Rats were separated into “No Anesthesia”, “5% Isoflurane”, or “200 mg/kg Sodium Pentobarbital” groups. For each group, animals were subjected to the indicated condition prior to rapid decapitation and pituitary isolation, after which pituitaries were flash frozen. Peptides were then extracted from pituitary samples and analyzed using an LC-MS and LC-MS/MS peptidomics workflow.
