Fig. 4. DUSP22 regulates the hepatic activation of NF-κB and FAK signaling in HFHC-fed mice.

a Representative western blotting and quantification of the protein expression of total and phosphorylated IKKα, IκBα, and NF-κB in liver of DUSP22^flox, DUSP22^HepKO, DUSP22^HepRosa, and DUSP22^HepOE mice fed with a NCD or a HFHC for 24 weeks (n = 4 mice per group) (*P < 0.05, **P < 0.01, and ***P < 0.001; ns no significant difference). b Representative western blotting and quantification of the protein expression of total and phosphorylated p38, MEK1/2, ERK1/2, and JNK1/2 in liver of DUSP22^flox, DUSP22^HepKO, DUSP22^HepRosa, and DUSP22^HepOE mice fed with a NCD or a HFHC for 24 weeks (n = 4 mice per group) (*P < 0.05, **P < 0.01, and ***P < 0.001; ns no significant difference). c Representative western blotting and quantification of the protein expression of total and phosphorylated FAK^Y576+Y577, FAK^Y397, ASK1, TAK1, and TBK1 in liver of DUSP22^flox, DUSP22^HepKO, DUSP22^HepRosa, and DUSP22^HepOE mice fed with a NCD or a HFHC for 24 weeks (n = 4 mice per group) (*P < 0.05, **P < 0.01, and ***P < 0.001; ns no significant difference). Data are expressed as mean ± SEM from at least three independent experiments. Statistical analysis was carried out by two-tailed Student’s t-test.