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. 2022 Oct 8;13(10):858. doi: 10.1038/s41419-022-05307-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Increased expression of PLEKHH2 was observed in A549 and H1299 cells transfected with the PLEKHH2-cDNA plasmid. Transfection efficiency was measured 48 h later via western blotting. B A significant increase in colony number was observed in cells with upregulated PLEKHH2 expression compared with the controls. C The CCK-8 assay showed a time-dependent increase in cell proliferation after PLEKHH2 plasmid transfection. D Flow cytometry results for the cell cycle analysis. Treatment with PLEKHH2 plasmids led to a significant decrease in the proportion of G-phase cells and a significant increase in S-phase cells compared with control cells. The scratch wound assay (E) and Transwell migration assay (F) revealed that cell migration was promoted in cells treated with the PLEKHH2 plasmid. G The Matrigel Transwell assay showed that cell invasion was significantly increased after PLEKHH2 transfection compared with the controls. H The results of the 3D invasion experiment showed that the invasion area of cells transfected with the PLEKHH2 plasmid was greater than that of the cells transfected with the control plasmid. I Cells transfected with PLEKHH2 plasmids showed increased expression of proliferation- and invasion-related proteins. P < 0.05 indicates statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001.