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. 2022 Oct 8;13(10):858. doi: 10.1038/s41419-022-05307-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A The SMART database was used to analyze the cellular functions of proteins containing the FERM domain. B The GSEA database predicted that PLEKHH2 was enriched in focal adhesion assemblies. C Overexpression of PLEKHH2 promotes FAK phosphorylation, which plays an important role in the focal adhesion assembly process. PLEKHH2 knockdown decreased FAK phosphorylation, but the total protein level of FAK did not change significantly. D After inhibiting the activity of the FAK via PF573228 treatment in A549 and H1299 cells, an increase in PLEKHH2 expression did not increase p-PI3K, p-AKT, or other proliferation-, migration-, and invasion-related proteins. Statistical analysis is shown in Supplementary Fig. 5A and 5B. Results of the clone formation assay (E, Statistical analysis is shown in Supplementary Fig. 5C and D), CCK-8 assay (F), and Matrigel Transwell assay (G, Statistical analysis is shown in Supplementary Fig. 5E and F) showed that, following the inhibition of FAK phosphorylation, the upregulation of PLEKHH2 did not significantly promote cell proliferation and invasion ability. P < 0.05 indicates statistical significance, *P < 0.05.