(A, B) A representative example of a nonconvulsive seizure in an Amigo2-Cre mouse expressing hM4D(Gi)-mCherry in CA2. Despite the absence of behavioral indicators (A), EEG revealed significant seizure activity in all electrodes (B). (C) CNO reduced nonconvulsive seizure frequency. (D) CNO did not alter seizure duration (paired t-test; t = 0.6743, df = 6; P = 0.5252; n = 8 mice). (E) CNO did not reduce nonconvulsive seizure frequency in two control groups of mice described in the previous figure (paired t-test; t = 0.1333, df = 11; P = 0.8963; n = 12 mice). (F) CNO did not reduce nonconvulsive seizure duration in control groups (paired t-test; t = 0.2927, df = 5; P = 0.7815; n = 6 mice).