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. Author manuscript; available in PMC: 2023 Oct 4.
Published in final edited form as: Cell Metab. 2022 Aug 29;34(10):1548–1560.e6. doi: 10.1016/j.cmet.2022.08.005

Figure 4. PIDD1 and RAIDD are needed for Casp2 activation.

Figure 4.

A-C. Parental (EVΔ), PIDD1- (PIDD1Δ) or RAIDD-ablated (RAIDDΔ) HEK293 cells were transfected with plasmids encoding epitope-tagged PIDDosome components, S1P, and SREBP2 as indicated and cultured for 16 hrs. Membrane and nuclear fractions were isolated and the indicated proteins were IB analyzed.

D. HEK293 cells were transfected with the indicated plasmids and supplemented with 25-hydroxycholesterol (25-HC, 1 μg/ml) for 16 hrs. Indicated proteins were IB analyzed. Cleaved S1P is shown in the red box.

Each experiment was repeated at least 3 times and representative results are shown. F-Full length, C-Cleaved, P-precursor, N.S-non-specific, NE-nuclear extract.