| Antibodies |
| Rat monoclonal anti-Casp2 |
Enzo Life Science |
Cat# ALX-804-356; RRID: AB_2275199 |
| Rabbit polyclonal anti-Casp2 |
Abcam |
Cat# Ab182657; RRID: |
| Mouse monoclonal anti-PIDD1 |
Adipogen |
Cat# AG-20B-0038-C100; RRID: |
| Rabbit polyclonal anti-PIDD1 |
Abcam |
Cat# Ab231315; RRID: |
| Rabbit polyclonal anti-RAIDD |
Proteintech |
Cat# 10401-1-AP; RRID:AB_2085477 |
| Mouse monoclonal anti-SREBP1 |
Abcam |
Cat# ab3259; RRID: AB_303650 |
| Rabbit polyclonal anti-SREBP2 |
Abcam |
Cat# ab30682; RRID:AB_779079 |
| Mouse monoclonal anti-S1P |
Santa Cruz Technologies |
Cat# sc271916; RRID: AB_10610623 |
| Rabbit polyclonal anti-INSIG2 |
ThermoFisher Scientific |
Cat# PA5-41707; RRID:AB_2576306 |
| Rabbit polyclonal anti-p-IRE1 |
Novos Biologicals, LLC. |
Cat# NB100-2323; RRID:AB_10145203 |
| Rabbit monoclonal anti-IRE1 |
Cell Signaling Technology |
Cat# 3294; RRID:AB_10830732 |
| Rabbit polyclonal anti-c/EBPbeta/LAP |
Cell Signaling Technology |
Cat# 3087; RRID:AB_2260365 |
| Rabbit polyclonal anti-ERK |
Cell Signaling Technology |
Cat#9 102; RRID: AB_330744 |
| Rabbit polyclonal anti-ACC |
Cell Signaling Technology |
Cat# 3662; RRID: AB_2219400 |
| Rabbit monoclonal anti-FAS |
Cell Signaling Technology |
Cat# 3180; RRID: AB_2100796 |
| Rabbit polyclonal anti-Hmgcr |
Santa Cruz Technologies |
Cat#s c33827; RRID:AB_2118193 |
| Rabbit monoclonal anti-PDI |
Cell Signaling Technology |
Cat# 3501; RRID: AB_2156433 |
| Rat monoclonal anti-HA |
Roche |
Cat# 1867431; RRID:AB_390918 |
| Rabbit polyclonal anti-Flag |
Sigma Aldrich |
Cat# F7425; RRID: AB_439687 |
| Mouse monoclonal anti-Tubulin |
Sigma Aldrich |
Cat# T5168; RRID: AB_477579 |
| Mouse monoclonal anti-Myc tag |
Abcam |
Cat# Ab32; RRID:AB_303599 |
| Rabbit monoclonal anti-V5 |
Cell Signaling Technology |
Cat# 13202; RRID:AB_2687461 |
| Bacterial and virus strains |
| OmniMax™ 2 T1R Chemically competent cells |
ThermoFisher Scientific |
C854003 |
| Chemicals, peptides, and recombinant proteins |
| 25-Hydroxycholesterol |
MedChemExpress |
Cat# HY-113134 |
| Puromycin |
InvivoGen |
Cat# Ant-pr-1 |
| Lipofectamine 3000 Transfection Reagent |
ThermoFisher Scientific |
Cat# L3000015 |
| MKC3946 |
EMD Millipore |
Cat#532758 |
| TRIzol Reagent |
Invitrogen |
Cat# 15596026 |
| PNGaseF |
New England BioLabs |
Cat#P0704 |
| EndoH |
New England BioLabs |
Cat#P0702 |
| Nhe1 |
New England BioLabs |
Cat#R3131S |
| BamH1 |
New England BioLabs |
Cat#R3136S |
| Not1 |
New England BioLabs |
Cat#R3189S |
| EcoR1 |
New England BioLabs |
Cat#R3101S |
| Critical commercial assays |
| Triglyceride Quantification Kit |
Sigma Aldrich |
Cat# MAK266 |
| Cholesterol Quantification Kit |
Sigma Aldrich |
Cat# MAK043 |
| Mouse INSULIN ELIZA Kit |
Invitrogen |
Cat# EMINS |
| ATP Assay Kit |
Abcam |
Cat# Ab83355 |
| Super Script VILO cDNA Synthesis Kit Scientific |
ThermoFisher Scientific |
Cat# 11754050 |
| Deposited data |
| RNAseq |
This paper |
PRJNA775619 |
|
Data S1_Source Data
|
This paper |
|
| Experimental models: Cell lines |
| HEK293 cells |
ATCC |
CRL-1573 |
| PIDD1-ablated HEK293 cells |
This paper |
N/A |
| RAIDD-ablated HEK293 cells |
This paper |
N/A |
| Experimental models: Organisms/strains |
| Mouse: Casp2 flox |
This paper |
N/A |
| Mouse: Casp2−/−
|
O’Reilly et al., 2002
|
N/A |
| Mouse: PIDD1−/−
|
Manzl et al., 2009
|
N/A |
| Mouse: RAIDD−/−
|
Berube et al., 2005
|
N/A |
| Mouse: SCAP flox |
Moon et al., 2011 |
N/A |
| Oligonucleotides |
| Guide sequence against human PIDD1, see experimental model and subject details
|
This paper |
N/A |
| Guide sequence against human CRADD, see experimental model and subject details
|
This paper |
N/A |
| Primers for generation of PIDD1-Flag, PIDD1ΔDD-Flag, RAIDD-6HIS, and V5-SREBP2, see experimental model and subject details
|
This paper |
N/A |
| Primers for analysis of mRNA analyses, see experimental model and subject details
|
This paper |
N/A |
| Recombinant DNA |
| pCDH-CMV-MCS-EF1-Puro |
System Bioscience |
Cat#CD500-CD700 |
| plentiCRISPRv2 vector |
Sanjana et al., 2014
|
N/A |
| pCMV6-Myc-DDK-Cradd |
OriGene Technologies, Inc. |
Cat#MR202001 |
| pCDH-CMV-MCS-EF1-Puro-mCasp2-HA |
Kim J.Y et al., Cell 2018
|
N/A |
| pCDH-CMV-MCS-EF1-Puro-mPIDD1-Flag |
This Paper |
N/A |
| pCDH-CMV-MCS-EF1-Puro-mRAIDD-6HIS |
This Paper |
N/A |
| pCDH-CMV-MCS-EF1-Puro-Myc-S1P |
Kim J.Y et al., Cell 2018
|
N/A |
| pCDH-CMV-MCS-EF1-Puro-V5-mSREBP2 |
This Paper |
N/A |
| pEGFP-SCAP |
Nohturfft et al., 2000 (PMID: 10975522) |
N/A |
| Software and algorithms |
| Illustrator |
Adobe |
http://www.adobe.com
|
| Photoshop |
Adobe |
http://www.adobe.com
|
| Image J |
Open Source/National Institute of Health |
http://imagej.nih.gov/ij/
|
| GraphPad Prizm 7.0 software |
GraphPad Software. Inc. |
http://www.graphpad.com/scientificsoftware/prism/ |
| Other |
| Diet: Normal chow diet |
LabDiet |
Rodent Diet 5053 |
| Diet: Cornstarch diet |
Research Diets, Inc. |
D12450K |
| Diet: Fructose diet |
Research Diets, Inc. |
D02022704K |
| Diet: High fat diet |
Bio-Serv |
S3282 |
| Diet: Western Diet |
ENVIGO |
TD88137 |
| Diet: D-(−)-Fructose |
Sigma Aldrich |
F3510 |
| Diet: D-(+)-Glucose |
Sigma Aldrich |
G5767 |
