TABLE 2.
Regulation of agmatine deiminase and N-carbamoylputrescine amidohydrolase synthesis
| Enzyme source | Supplementsa | Sp act
(U/mg of protein)b
|
|
|---|---|---|---|
| Agmatine deiminase | N-Carbamoylputrescine amidohydrolase | ||
| PAO1 (wild type) | Suc + NH4 | 2 ± 0 | 3 ± 1 |
| Glu | 3 ± 1 | 2 ± 1 | |
| Agm | 50 ± 3 | 17 ± 3 | |
| N-CP | 26 ± 2 | 8 ± 0 | |
| Glu + Agm | 31 ± 2 | 11 ± 1 | |
| Glu + N-CP | 22 ± 0 | 6 ± 2 | |
| Suc + Agm | 25 ± 1 | 8 ± 0 | |
| Suc + N-CP | 21 ± 0 | 5 ± 1 | |
| PAO4151 (aguA9001) | Glu | <1 | 2 ± 1 |
| Glu + Agm | <1 | 11 ± 4 | |
| Glu + N-CP | <1 | 5 ± 0 | |
| PAO4179 (aguB9001) | Glu | 2 ± 1 | <1 |
| Glu + Agm | 42 ± 5 | <1 | |
| Glu + N-CP | 26 ± 2 | 1 ± 0 | |
| PAO4495 (aguR::ΩSp/Sm) | Suc + NH4 | 52 ± 4 | 19 ± 2 |
| Glu | 56 ± 2 | 20 ± 1 | |
| Agm | 73 ± 7 | 25 ± 1 | |
| N-CP | 57 ± 3 | 20 ± 2 | |
a
Cells were grown to an optical density at 660 nm of 0.5 in MMP containing the indicated supplements as the C and N sources at 20 mM. Cell extracts and enzyme assays were prepared as described in Materials and Methods. Agm, agmatine; Glu, glutamate; N-CP, N-carbamoylputrescine; Suc, succinate.
b
Values are averages of three independent measurements with standard errors.