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. 2001 Nov;183(22):6525–6531. doi: 10.1128/JB.183.22.6525-6531.2001

FIG. 1.

FIG. 1

Effect of suppression of pbp2 transcription on growth of MRSA strain COL and MSSA strain RN4220. (a) Bacterial cultures grown on solid medium (TSA) supplemented or not supplemented with IPTG. Sector A, COLpPBP2iII-1; Sector B, COLpPBP2iII-5; Sector C, RNpPBP2iII-1; Sector D, RNpPBP2iII-5. (b) Northern blot hybridization with probe for pbp2. Lanes A, COLpPBP2iII-1 lanes B, COLpPBP2iII-5. Lane COL contained wild-type strain COL. RNA was prepared from cultures grown with or without 0.5 mM IPTG.