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Effect of PBP2 deficit on the muropeptide composition of the cell walls of MRSA strain COL. We determined the HPLC profiles of COLpPBP2iII-5 grown in the presence (A) and in the absence (B) of IPTG, as well as the HPLC profile of strain COL grown in the presence of 1 μg of oxacillin per ml (C). Muropeptides were purified and separated by HPLC as described in the text.
