Fig. 5.

HD model Hdh^Q140 transgenic mice appeared HD-associated biomarker and phenotypes. A–B The expression of T-HTT (anti-HTT, MAB2166) and DARPP-32 in the striatum of 12-month-old mice for homozygous Hdh^Q140/Q140 (A), and the protein level were quantified (B). Data are presented as mean ± sem from three individual samples. C Recognition index of wild-type mice, heterozygote, and homozygous Hdh^Q140 mice in novel object recognition test at 12-month-old. D–F Total distance (D), central /peripheral ratio (E), and times of lifting at least two limps in open-field test in 15 min (F) at 12-month-old. G–J Balance beam test of the slip faults in 15 mm square (G), 15 mm round (H), 10 mm square (I), and 10 mm round (J). K–M Rotarod assay of the latency time to fall at speed of 4 rpm (K), 8 rpm (L), 4 ~ 8 rpm (M) in 2 min. N The evaluation scores of mice in climbing test. Each dot represented an individual mouse, and bar represented mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns means not significant