TABLE 1.
Analysis of L11-RelA interaction in the yeast two-hybrid systema
| Transformant | GAL4 (DB) plasmid used | GAL4 (AD) plasmid used | Growth on SD selection medium | β-Galactosidase activity (Miller units)b |
|---|---|---|---|---|
| 1 | pGBKT7 (cloning vector) | pGADT7 (cloning vector) | − | 0.3 ± 0.1 |
| 2 | pGBKT7-53 (murine p53) | pGADT7-T (SV40 T antigen) | + | 18.8 ± 2.2 |
| 3 | pXY62 (′RelA) | pXY48 (L11) | − | 0.5 ± 0.4 |
| 4 | pXY49 (L11) | pXY63 (′RelA) | − | 0.6 ± 0.3 |
a
DB, DNA-binding; AD, activation domain.
b
Average of three independent determinations