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AKG prevented TNF-α-caused synthetic phenotype acquisition in vitro. A Representative image and quantification of VSMCs proliferation by Ki-67 staining (n = 5). B Typical image as well as quantitative analysis on VSMCs invasion by wound scratch assay (n = 4). C Typical image as well as quantitative analysis on VSMCs migration by transwell assay (n = 4). D The active MMP-2 (68 kDa) and SM22α expression were analyzed by western blot (n = 4). *P < 0.05
