Fig. 7.

Lentivirus-mediated PXDN up-regulation reduced AKG’s protection in vitro. A The PXDN expression were analyzed by western blot (n = 4). B MCP-1, IL-1β and IL-6 expression was measured using ELISA (n = 5). C Typical images as well as quantitative analysis on superoxide anion expression measured through DCFH-DA staining (n = 5). D Typical images as well as quantitative analysis on VSMCs migration by transwell assay (n = 4). E The active MMP-2 (68 kDa), SM-22α and NOX2 expression were examined through WB assay (n = 4). *P < 0.05, **P < 0.01