Fig. 2.

Agarose gel electrophoresis shows the expression of histamine receptors H2R (A, B), H3R (C, D), and H4R (E, F) genes on mouse cumulus oocyte complex (COCs) and cumulus free oocytes (CFO). M: 100 bp DNA ladder. A) Lanes 1-3: ACTB on COC (539 bp); Lanes 4-6: H2R on COC (442 bp); Lane 7: Negative control. B) Lanes 1-3: H2R on stomach (610 bp); Lanes 4-6: H2R on CFO (610 bp); Lane 7: Negative control. C) Lane 1: Negative control; Lane 2: ACTB on COC (539 bp); Lanes 3-5: H3R on brain (282 bp); Lanes 6-8: H3R on COC (282 bp). D) Lanes 1-3: ACTB on brain (539 bp); Lanes 4-6: ACTB on CFO (539 bp); Lanes 7-9: H3R on brain (282 bp); Lanes 10-12: H3R on CFO (282 bp); Lane 13: Negative control. E) Lanes 1 and 2: H4R on COC; Lane 3: Negative control; Lanes 4-6: H4R on testis (806 bp). F) Lanes 1-3: H4R on testis (806 bp); Lane 4: H4R on CFO; Lane 5: Negative control