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. Author manuscript; available in PMC: 2023 Oct 4.
Published in final edited form as: Biochemistry. 2022 Sep 13;61(19):2106–2117. doi: 10.1021/acs.biochem.2c00354

Figure 4.

Figure 4.

Size exclusion chromatography of iron-loaded ferritin in the presence of phosphate (straight lines) and of Fe(III)–phosphate complexes (dotted lines) used as controls. Conditions: (A) 0.2 μM H-ferritin and (B) 0.2 μM L-ferritin, 40 μM Fe(II) (or 200 Fe/shell), in 50 mM Mops, 100 mM NaCl, pH 7.4, and 0, 1, 5, or 10 mM phosphate.