TABLE 1.
List of E. coli K-12 strainsa
| Strain | Mutation(s) |
|---|---|
| GJ11 | proU224::lac |
| GJ862 | Nil |
| GJ863 | rho |
| GJ866 | hns |
| GJ867 | rho hns |
| GJ870 | rpoS |
| GJ871 | rho rpoS |
| GJ872 | hns rpoS |
| GJ873 | rho hns rpoS |
| GJ884 | csiD::lac |
| GJ885 | csiD::lac rho |
| GJ887 | csiD::lac rho rpoS |
| GJ888 | osmY::lac |
| GJ889 | osmY::lac rho |
| GJ891 | osmY::lac rho rpoS |
| GJ2733 | csiD::lac rpoS |
| GJ2734 | osmY::lac rpoS |
| GJ2739 | csiD::lac hns |
| GJ2741 | osmY::lac hns |
| GJ2743 | proU224::lac rpoS |
| GJ2745 | hfq-2 |
| GJ2746 | hfq-1 |
| GJ2748 | hfq-1 hns |
| GJ2750 | csiD::lac hns rpoS |
| GJ2751 | osmY::lac hns rpoS |
| GJ2752 | hfq-1 hns rpoS |
All listed strains are F− and, with the exception of GJ11 (15), were constructed in this study. All strains are derivatives of MC4100 (15), and mutations for each in addition to those present in this strain are listed. Genotype designations are as in the work of Berlyn (6). The complete genotype of MC4100 is Δ(argF-lac)U169 rpsL 150 relA1 araD139 flbB5301 deoC1 ptsF25. Allele numbers of the listed mutations and the strains (sources) from which they were transferred by P1 transduction are as follows: rho, rho-4(Am) from CGSC5072 (E. coli Genetic Stock Center); hns, hns-206::Ap from PD32 (12); rpoS, rpoS359::Tn10 from RH90 (5); csiD::lac, csiD::λplacMu15 from DW12 (5); osmY::lac (previously called csi-5::lac), osmY::λplacMu55 from RO151-a (5); hfq-1, hfq-1::Ω from TX2822 (M. E. Winkler strain derived from TX2808 [53]); and hfq-2, hfq-2::Ω from TX2765 (M. E. Winkler strain derived from TX2758 [53]).