TABLE 2.
proU P1-lac expression at 30 and 10°Ca
| Temp and GJ rpoS+ strain no.d | Mutation(s) | β-Galactosidase sp act for P1-lac-bearing plasmid (description) on rpoS+ strainb
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pHYD373 (S. enterica P1)
|
pHYD374 (S. enterica P1Δatt)
|
pHYD275 (E. coli P1.a)
|
pHYD380 (E. coli P1.b)
|
pHYD394 (E. coli P1-P2∗- NRE.a)
|
pHYD395 (E. coli P1-P2∗- NRE.b)
|
||||||||
| −NaCl | +NaCl | −NaCl | +NaCl | −NaCl | +NaCl | −NaCl | +NaCl | −NaCl | +NaCl | −NaCl | +NaCl | ||
| 30°C | |||||||||||||
| 862 (870) | Nil | 4 (<1) | 4 (<1) | 60 (2) | 111 (<1) | 63 (4) | 275 (8) | 51 (5) | 277 (8) | 3 (2) | 6 (3) | 2 (1) | 6 (3) |
| 863 (871) | rho | 51 (<1) | 69 (3) | 136 (5) | 159 (4) | 126 (15) | 322 (18) | 73 (13) | 291 (16) | 4 (3) | 11 (4) | 3 (2) | 8 (6) |
| 866 (872) | hns | 5 (3) | 7 (2) | 391 (3) | 301 (4) | 407 (16) | 620 (22) | 349 (35) | NDc | 75 (8) | 112 (14) | 58 (10) | 168 (15) |
| 867 (873) | rho hns | 58 (5) | 82 (2) | 472 (7) | 331 (5) | 393 (27) | 500 (38) | ND | ND | 106 (27) | 207 (41) | 80 (23) | 251 (35) |
| 10°C | |||||||||||||
| 862 (870) | Nil | 54 (2) | 22 (2) | 728 (6) | 288 (4) | 962 (16) | 665 (16) | ND | ND | 5 (4) | 8 (7) | 5 (1) | 10 (1) |
| 863 (871) | rho | 463 (35) | 277 (20) | 917 (67) | 427 (38) | 885 (69) | 667 (47) | ND | ND | 114 (17) | 136 (13) | 136 (22) | 175 (13) |
a
Isogenic rpoS+ and rpoS cultures were grown in trimethoprim-supplemented K-tryptone medium without NaCl (−NaCl) or with 0.3 M NaCl (+NaCl) at 30 or 10°C for β-galactosidase assays. Enzyme specific activity values are reported in Miller units (35). Values in parentheses are for rpoS derivatives.
b
Plasmid descriptions are as explained in Fig. 1 and its legend.
c
ND, not determined.
d
Strain numbers outside and within parentheses designate isogenic rpoS+ and rpoS derivatives, respectively.